SpiderP

SpiderP first runs SignalP to detect a signal peptide, then uses a cleavage window model (with empirical motif reconciliation) to predict propeptide boundaries and the mature toxin region.

Input requirements

• Standard amino-acid letters only (A–Z, X allowed for unknown residues).
• At least 10 amino acids after any FASTA header is removed.
• Paste raw sequence or FASTA (>name on the first line). Header-only rows are rejected.

Choose Single Sequence or Batch Processing (Excel) at the top of the form.

Single Sequence

• Paste a protein sequence with or without a FASTA header (>name on the first line).
• Click SpiderP. Processing runs in the background; progress appears on this page.
• Invalid input is rejected before processing starts, with an error message.
• Use Cancel Job to stop a running job, or Refresh to clear the form.

Batch Processing (Excel)

• Upload an Excel or CSV file (.xlsx, .xls, .csv).
• Put sequences in a column named Sequence (or use the first column).
• Optionally add a Header, ID, or Name column, or put a FASTA header (>…) on the row above each sequence.
• Rows that fail validation are skipped; you will see how many were skipped and sample row numbers.
• Click Process Batch. A summary table appears when all jobs finish; an Excel file also downloads automatically.

Results list predicted Signal Peptide, Propeptide, and Mature Toxin segments with start and end positions. The full input sequence is color-coded below the table: purple = signal, green = propeptide, red = mature toxin.

If SignalP finds no signal peptide, SpiderP still completes but shows a warning (not a failure): the entire sequence is treated as mature. Warnings also appear when the predicted mature region looks atypical for spider toxins (unusual length or cysteine count). Review these manually before relying on the result.

Use Download Results (Excel) to save single or batch output. Toxin cards can pre-fill the single-sequence form via their SpiderP links.