Run BLAST against Local Database

Paste a query under Enter query sequence, or upload a FASTA file (.fasta, .fa, .txt). You may submit one or many sequences — use multi-record FASTA (each record starting with >) or a single raw sequence. Examples:

• Raw: MKFFLLFLVVLPIMGALGKK...
• Single FASTA: >sequence_name on one line, then the sequence
• Multi-FASTA: multiple >header / sequence blocks (up to 50 queries per job)

Set Query sequence type and BLAST against to a matching pair:

• Protein query → Peptide Sequences (blastp)
• DNA query → Nucleic Sequences (blastn)

Open Algorithm parameters to adjust the E-value threshold (default 10; lower is stricter) and the maximum number of hits to return (1–10,000).

Click BLAST to submit the job. A status page tracks progress and redirects automatically when the search finishes. You can bookmark the job URL to check it later.

On the results page, the Descriptions table lists bit score, E-value, identity, and gaps. Matching toxin names link to toxin cards in a new tab. The Alignments section shows each hit in detail; use per-row Download or Download Results for the full report.

Use New Search to run another query, or Refresh on this page to clear the form. Toxin cards can also pre-fill this form via their BLAST links.